Enhanced Validation

Anti-GALNT3 Antibody

polypeptide N-acetylgalactosaminyltransferase 3
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human GALNT3
Alternative Gene Names
GalNAc-T3, HFTC, HHS
Price
$445.00
Product Number
HPA007613
Unit Size
100 µl
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
polypeptide N-acetylgalactosaminyltransferase 3
Target Gene
GALNT3
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
EESRMERNMKNKNKMLDLMLEAVNNIKDAMPKMQIGAPVRQNIDAGERPCLQGYYTAAELKPVLDRPPQDSNAPGASGKAFKTTNLSVEEQKEKERGEAKHC
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000005727 (87%)

Mouse ENSMUSG00000026994 (86%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
polypeptide N-acetylgalactosaminyltransferase 3
Gene Name
GALNT3
Alternative Gene Names
GalNAc-T3, HFTC, HHS
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000115339 on the Human Protein Atlas

Stevenson NL, Bergen DJ, Skinner RE, Kague E, Martin-Silverstone E, Robson Brown KA, Hammond CL, Stephens DJ

Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression

J Cell Sci , 2017 Dec 15; 130(24):4132-4143

PubMed ID: 29093022 DOI: 10.1242/jcs.212308

Lorenz V, Ditamo Y, Cejas RB, Carrizo ME, Bennett EP, Clausen H, Nores GA, Irazoqui FJ

Extrinsic Functions of Lectin Domains in O-N-Acetylgalactosamine Glycan Biosynthesis

J Biol Chem , 2016 Oct 13; 291(49):25339-25350. Epub 2016 Oct 13

PubMed ID: 27738109 DOI: 10.1074/jbc.M116.740795

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Corresponding Antigens

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