Enhanced Validation

Anti-FUS Antibody

FUS RNA binding protein
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human FUS
Alternative Gene Names
ALS6, FUS1, hnRNP-P2, HNRNPP2, TLS
Price
$445.00
Product Number
HPA008784
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
FUS RNA binding protein
Target Gene
FUS
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
SSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDR
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000023360 (91%)

Mouse ENSMUSG00000030795 (91%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
FUS RNA binding protein
Gene Name
FUS
Alternative Gene Names
ALS6, FUS1, hnRNP-P2, HNRNPP2, TLS
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000089280 on the Human Protein Atlas

Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J

Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses

Front Cell Neurosci , 2019 Jun 12; 13:256. Epub 2019 Jun 12

PubMed ID: 31244613 DOI: 10.3389/fncel.2019.00256

Mateju D, Franzmann TM, Patel A, Kopach A, Boczek EE, Maharana S, Lee HO, Carra S, Hyman AA, Alberti S

An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function

EMBO J , 2017 Apr 4; 36(12):1669-1687. Epub 2017 Apr 4

PubMed ID: 28377462 DOI: 10.15252/embj.201695957

Kawakami I, Kobayashi Z, Arai T, Yokota O, Nonaka T, Aoki N, Niizato K, Oshima K, Higashi S, Katsuse O, Hosokawa M, Hasegawa M, Akiyama H

Chorea as a clinical feature of the basophilic inclusion body disease subtype of fused-in-sarcoma-associated frontotemporal lobar degeneration

Acta Neuropathol Commun , 2016 April 4; 4:36. Epub 2016 Apr 4

PubMed ID: 27044537 DOI: 10.1186/s40478-016-0304-9

Schoen M, Reichel JM, Demestre M, Putz S, Deshpande D, Proepper C, Liebau S, Schmeisser MJ, Ludolph AC, Michaelis J, Boeckers TM

Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons

Front Cell Neurosci , 2016 Jan 12; 9:496. Epub 2016 Jan 12

PubMed ID: 26834559 DOI: 10.3389/fncel.2015.00496

Ziskin JL, Greicius MD, Zhu W, Okumu AN, Adams CM, Plowey ED

Neuropathologic analysis of Tyr69His TTR variant meningovascular amyloidosis with dementia

Acta Neuropathol Commun , 2015 Jul 10; 3:43. Epub 2015 Jul 10

PubMed ID: 26156087 DOI: 10.1186/s40478-015-0216-0

Barmada SJ, Ju S, Arjun A, Batarse A, Archbold HC, Peisach D, Li X, Zhang Y, Tank EM, Qiu H, Huang EJ, Ringe D, Petsko GA, Finkbeiner S

Amelioration of toxicity in neuronal models of amyotrophic lateral sclerosis by hUPF1

Proc Natl Acad Sci U S A , 2015 Jun 23; 112(25):7821-7826. Epub 2015 Jun 8

PubMed ID: 26056265 DOI: 10.1073/pnas.1509744112

Nakamura M, Bieniek KF, Lin WL, Graff-Radford NR, Murray ME, Castanedes-Casey M, Desaro P, Baker MC, Rutherford NJ, Robertson J, Rademakers R, Dickson DW, Boylan KB

A truncating SOD1 mutation, p.Gly141X, is associated with clinical and pathologic heterogeneity, including frontotemporal lobar degeneration

Acta Neuropathol , 2015 Apr 28; 130(1):145-157. Epub 2015 Apr 28

PubMed ID: 25917047 DOI: 10.1007/s00401-015-1431-2

Ferrer I, Legati A, García-Monco JC, Gomez-Beldarrain M, Carmona M, Blanco R, Seeley WW, Coppola G

Familial Behavioral Variant Frontotemporal Dementia Associated With Astrocyte-Predominant Tauopathy

J Neuropathol Exp Neurol , 2015/04/01; 74(4):370-379

PubMed ID: 25756587 DOI: 10.1097/NEN.0000000000000180

Ayaki T, Ito H, Fukushima H, Inoue T, Kondo T, Ikemoto A, Asano T, Shodai A, Fujita T, Fukui S, Morino H, Nakano S, Kusaka H, Yamashita H, Ihara M, Matsumoto R, Kawamata J, Urushitani M, Kawakami H, Takahashi R

Immunoreactivity of valosin-containing protein in sporadic amyotrophic lateral sclerosis and in a case of its novel mutant

Acta Neuropathol Commun , 2014 Dec 10; 2:172. Epub 2014 Dec 10

PubMed ID: 25492614 DOI: 10.1186/s40478-014-0172-0

Sephton CF, Tang AA, Kulkarni A, West J, Brooks M, Stubblefield JJ, Liu Y, Zhang MQ, Green CB, Huber KM, Huang EJ, Herz J, Yu G

Activity-dependent FUS dysregulation disrupts synaptic homeostasis

Proc Natl Acad Sci U S A , 2014 Nov 4; 111(44):E4769-E4778. Epub 2014 Oct 16

PubMed ID: 25324524 DOI: 10.1073/pnas.1406162111

Nakamura M, Murray ME, Lin WL, Kusaka H, Dickson DW

Optineurin immunoreactivity in neuronal and glial intranuclear inclusions in adult-onset neuronal intranuclear inclusion disease

Am J Neurodegener Dis , 2014; 3(2):93-102. Epub 2014 Sep 6

PubMed ID: 25232514

Qiu H, Lee S, Shang Y, Wang WY, Au KF, Kamiya S, Barmada SJ, Finkbeiner S, Lui H, Carlton CE, Tang AA, Oldham MC, Wang H, Shorter J, Filiano AJ, Roberson ED, Tourtellotte WG, Chen B, Tsai LH, Huang EJ

ALS-associated mutation FUS-R521C causes DNA damage and RNA splicing defects

J Clin Invest , 2014 Mar; 124(3):981-999. Epub 2014 Feb 10

PubMed ID: 24509083 DOI: 10.1172/JCI72723

Dobson-Stone C, Luty AA, Thompson EM, Blumbergs P, Brooks WS, Short CL, Field CD, Panegyres PK, Hecker J, Solski JA, Blair IP, Fullerton JM, Halliday GM, Schofield PR, Kwok JB

Frontotemporal dementia–amyotrophic lateral sclerosis syndrome locus on chromosome 16p12.1–q12.2: genetic, clinical and neuropathological analysis

Acta Neuropathol , 2013 Apr; 125(4):523-533. Epub 2013 Jan 22

PubMed ID: 23338750 DOI: 10.1007/s00401-013-1078-9

Dormann D, Madl T, Valori CF, Bentmann E, Tahirovic S, Abou-Ajram C, Kremmer E, Ansorge O, Mackenzie IR, Neumann M, Haass C

Arginine methylation next to the PY-NLS modulates Transportin binding and nuclear import of FUS

EMBO J , 2012 Nov 14; 31(22):4258-4275. Epub 2012 Sep 11

PubMed ID: 22968170 DOI: 10.1038/emboj.2012.261

Snowden JS, Rollinson S, Thompson JC, Harris JM, Stopford CL, Richardson AM, Jones M, Gerhard A, Davidson YS, Robinson A, Gibbons L, Hu Q, DuPlessis D, Neary D, Mann DM, Pickering-Brown SM

Distinct clinical and pathological characteristics of frontotemporal dementia associated with C9ORF72 mutations

Brain , 2012 Mar; 135(3):693-708. Epub 2012 Feb 2

PubMed ID: 22300873 DOI: 10.1093/brain/awr355

Pikkarainen M, Hartikainen P, Soininen H, Alafuzoff I

Distribution and pattern of pathology in subjects with familial or sporadic late-onset cerebellar ataxia as assessed by p62/sequestosome immunohistochemistry.

Cerebellum , 2011 Dec; 10(4):720-31

PubMed ID: 21544590 DOI: 10.1007/s12311-011-0281-2

Neumann M, Bentmann E, Dormann D, Jawaid A, DeJesus-Hernandez M, Ansorge O, Roeber S, Kretzschmar HA, Munoz DG, Kusaka H, Yokota O, Ang LC, Bilbao J, Rademakers R, Haass C, Mackenzie IR

FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations

Brain , 2011 Sep; 134(9):2595-2609. Epub 2011 Aug 19

PubMed ID: 21856723 DOI: 10.1093/brain/awr201

Lashley T, Rohrer JD, Bandopadhyay R, Fry C, Ahmed Z, Isaacs AM, Brelstaff JH, Borroni B, Warren JD, Troakes C, King A, Al-Saraj S, Newcombe J, Quinn N, Ostergaard K, Schrøder HD, Bojsen-Møller M, Braendgaard H, Fox NC, Rossor MN, Lees AJ, Holton JL, Revesz T

A comparative clinical, pathological, biochemical and genetic study of fused in sarcoma proteinopathies

Brain , 2011 Sep; 134(9):2548-2564. Epub 2011 Jul 12

PubMed ID: 21752791 DOI: 10.1093/brain/awr160

Kino Y, Washizu C, Aquilanti E, Okuno M, Kurosawa M, Yamada M, Doi H, Nukina N

Intracellular localization and splicing regulation of FUS/TLS are variably affected by amyotrophic lateral sclerosis-linked mutations

Nucleic Acids Res , 2011 Apr; 39(7):2781-2798. Epub 2010 Nov 24

PubMed ID: 21109527 DOI: 10.1093/nar/gkq1162

Urwin H, Josephs KA, Rohrer JD, Mackenzie IR, Neumann M, Authier A, Seelaar H, Van Swieten JC, Brown JM, Johannsen P, Nielsen JE, Holm IE, The FReJA Consortium, Dickson DW, Rademakers R, Graff-Radford NR, Parisi JE, Petersen RC, Hatanpaa KJ, White III CL, Weiner MF, Geser F, Van Deerlin VM, Trojanowski JQ, Miller BL, Seeley WW, van der Zee J, Kumar-Singh S, Engelborghs S, De Deyn PP, Van Broeckhoven C, Bigio EH, Deng HX, Halliday GM, Kril JJ, Munoz DG, Mann DM, Pickering-Brown SM, Doodeman V, Adamson G, Ghazi-Noori S, Fisher EM, Holton JL, Revesz T, Rossor MN, Collinge J, Mead S, Isaacs AM

FUS pathology defines the majority of tau- and TDP-43-negative frontotemporal lobar degeneration

Acta Neuropathol , 2010 Jul; 120(1):33-41. Epub 2010 May 20

PubMed ID: 20490813 DOI: 10.1007/s00401-010-0698-6

Deng HX, Zhai H, Bigio EH, Yan J, Fecto F, Ajroud K, Mishra M, Ajroud-Driss S, Heller S, Sufit R, Siddique N, Mugnaini E, Siddique T

FUS-immunoreactive inclusions are a common feature in sporadic and non-SOD1 familial amyotrophic lateral sclerosis

Ann Neurol , 2010 Jun; 67(6):739-748

PubMed ID: 20517935 DOI: 10.1002/ana.22051

Seelaar H, Klijnsma KY, de Koning I, van der Lugt A, Chiu WZ, Azmani A, Rozemuller AJ, van Swieten JC

Frequency of ubiquitin and FUS-positive, TDP-43-negative frontotemporal lobar degeneration.

J Neurol , 2010 May; 257(5):747-53. Epub 2009 Nov 28

PubMed ID: 19946779 DOI: 10.1007/s00415-009-5404-z

Neumann M, Roeber S, Kretzschmar HA, Rademakers R, Baker M, Mackenzie IR

Abundant FUS-immunoreactive pathology in neuronal intermediate filament inclusion disease

Acta Neuropathol , 2009 Nov; 118(5):605-616. Epub 2009 Aug 9

PubMed ID: 19669651 DOI: 10.1007/s00401-009-0581-5

Neumann M, Rademakers R, Roeber S, Baker M, Kretzschmar HA, Mackenzie IR

A new subtype of frontotemporal lobar degeneration with FUS pathology

Brain , 2009 Nov; 132(11):2922-2931. Epub 2009 Aug 12

PubMed ID: 19674978 DOI: 10.1093/brain/awp214

Munoz DG, Neumann M, Kusaka H, Yokota O, Ishihara K, Terada S, Kuroda S, Mackenzie IR

FUS pathology in basophilic inclusion body disease.

Acta Neuropathol , 2009 Nov; 118(5):617-27. Epub 2009 Oct 15

PubMed ID: 19830439 DOI: 10.1007/s00401-009-0598-9

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Alternative Antibodies
Corresponding Antigens

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