Enhanced Validation

Anti-FSCN1 Antibody

fascin actin-bundling protein 1
Recommended Applications
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human FSCN1
Alternative Gene Names
FLJ38511, p55, SNL
Price
$445.00
Product Number
HPA005723
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
fascin actin-bundling protein 1
Target Gene
FSCN1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQ
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000029581 (96%)

Rat ENSRNOG00000056585 (63%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-FSCN1 HPA050654.

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
fascin actin-bundling protein 1
Gene Name
FSCN1
Alternative Gene Names
FLJ38511, p55, SNL
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000075618 on the Human Protein Atlas

Jacobs-Cachá C, Torres IB, López-Hellín J, Cantarell C, Azancot MA, Román A, Moreso F, Serón D, Meseguer A, Sarró E

Fascin-1 is released from proximal tubular cells in response to calcineurin inhibitors (CNIs) and correlates with isometric vacuolization in kidney transplanted patients

Am J Transl Res , 2017 Sep 15; 9(9):4173-4183. Epub 2017 Sep 15

PubMed ID: 28979691

Kliewe F, Scharf C, Rogge H, Darm K, Lindenmeyer MT, Amann K, Cohen CD, Endlich K, Endlich N

Studying the role of fascin-1 in mechanically stressed podocytes

Sci Rep , 2017 Aug 30; 7:9916. Epub 2017 Aug 30

PubMed ID: 28855604 DOI: 10.1038/s41598-017-10116-4

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Alternative Antibodies
Corresponding Antigens

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