Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000000875 (91%)
Mouse ENSMUSG00000023092 (90%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Molecular autopsy and family screening in a young case of sudden cardiac death reveals an unusually severe case of FHL1 related hypertrophic cardiomyopathy
Mol Genet Genomic Med , 2019 Jul 10; 7(8):e841. Epub 2019 Jul 10
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Mol Cell Proteomics , 2006 Jun; 5(6):1072-81. Epub 2006 Mar 8
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