Enhanced Validation

Anti-FBN1 Antibody

fibrillin 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human FBN1
Alternative Gene Names
FBN, MASS, MFS1, OCTD, SGS, WMS
Price
$445.00
Product Number
HPA021057
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target Protein
fibrillin 1
Target Gene
FBN1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DVRPGYCYTALTNGRCSNQLPQSITKMQCCCDAGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRYLCQNGRCIPTPGSCRCEC
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000007302 (89%)

Mouse ENSMUSG00000027204 (89%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
fibrillin 1
Gene Name
FBN1
Alternative Gene Names
FBN, MASS, MFS1, OCTD, SGS, WMS
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000166147 on the Human Protein Atlas

Cain SA, Mularczyk EJ, Singh M, Massam-Wu T, Kielty CM

ADAMTS-10 and -6 differentially regulate cell-cell junctions and focal adhesions

Sci Rep , 2016 Oct 25; 6:35956. Epub 2016 Oct 25

PubMed ID: 27779234 DOI: 10.1038/srep35956

Baldwin AK, Cain SA, Lennon R, Godwin A, Merry CL, Kielty CM

Epithelial–mesenchymal status influences how cells deposit fibrillin microfibrils

J Cell Sci , 2014 Jan 1; 127(1):158-171

PubMed ID: 24190885 DOI: 10.1242/jcs.134270

Zhang W, Ota T, Shridhar V, Chien J, Wu B, Kuang R

Network-based Survival Analysis Reveals Subnetwork Signatures for Predicting Outcomes of Ovarian Cancer Treatment

PLoS Comput Biol , 2013; 9(3):e1002975. Epub 2013 Mar 21

PubMed ID: 23555212 DOI: 10.1371/journal.pcbi.1002975

Edlund K, Lindskog C, Saito A, Berglund A, Pontén F, Göransson-Kultima H, Isaksson A, Jirström K, Planck M, Johansson L, Lambe M, Holmberg L, Nyberg F, Ekman S, Bergqvist M, Landelius P, Lamberg K, Botling J, Ostman A, Micke P

CD99 is a novel prognostic stromal marker in non-small cell lung cancer.

Int J Cancer , 2012 Nov 15; 131(10):2264-73. Epub 2012 Apr 24

PubMed ID: 22392539 DOI: 10.1002/ijc.27518

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