Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000003808 (90%)
Rat ENSRNOG00000003149 (90%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics
Mol Cell Proteomics , 2009 Jul; 8(7):1612-1622. Epub 2009 Apr 7
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.