Enhanced Validation

Anti-ESR1 Antibody

estrogen receptor 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
Product Description
Polyclonal Antibody against Human ESR1
Alternative Gene Names
Era, ESR, NR3A1
Price
$450.00
Product Number
HPA000450
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
estrogen receptor 1
Target Gene
ESR1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
LQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYIT
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000019358 (89%)

Mouse ENSMUSG00000019768 (89%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-ESR1 HPA000449.

Western Blot (WB)

Recombinant expression validation in WB using target protein overexpression.

Protein Name
estrogen receptor 1
Gene Name
ESR1
Alternative Gene Names
Era, ESR, NR3A1
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000091831 on the Human Protein Atlas

Lopez Sanchez MI, Shearwood AM, Chia T, Davies SM, Rackham O, Filipovska A

Estrogen-Mediated Regulation of Mitochondrial Gene Expression

Mol Endocrinol , 2014 Nov 6; 29(1):14-27. Epub 2014 Nov 6

PubMed ID: 25375021 DOI: 10.1210/me.2014-1077

Algenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S

Antibody performance in western blot applications is context-dependent.

Biotechnol J , 2014 Mar; 9(3):435-45. Epub 2014 Jan 29

PubMed ID: 24403002 DOI: 10.1002/biot.201300341

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Corresponding Antigens

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