Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000004078 (98%)
Mouse ENSMUSG00000060600 (96%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Enhancer hijacking activates oncogenic transcription factor NR4A3 in acinic cell carcinomas of the salivary glands
Nat Commun , 2019 Jan 21; 10:368. Epub 2019 Jan 21
The human cardiac and skeletal muscle proteomes defined by transcriptomics and antibody-based profiling
BMC Genomics , 2015 Jun 25; 16(1):475. Epub 2015 Jun 25
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
Proteome Sci , 2011 Nov 17; 9:73. Epub 2011 Nov 17
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Mol Cell Proteomics , 2006 Jun; 5(6):1072-81. Epub 2006 Mar 8
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