Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000044393 (65%)
Rat ENSRNOG00000016526 (59%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Upregulation of desmoglein 2 and its clinical value in lung adenocarcinoma: a comprehensive analysis by multiple bioinformatics methods
PeerJ , 2020 Feb 13; 8:e8420. Epub 2020 Feb 13
Intercalated discs: multiple proteins perform multiple functions in non-failing and failing human hearts.
Biophysical Reviews , 2009; 1(1):43-49
Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma.
Br J Dermatol , 2008 Mar; 158(3):527-38. Epub 2008 Jan 30
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