Enhanced Validation

Anti-DRAM2 Antibody

DNA-damage regulated autophagy modulator 2
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human DRAM2
Alternative Gene Names
MGC54289, PRO180, RP5-1180E21.1, TMEM77, WWFQ154
Price
$445.00
Product Number
HPA018036
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
DNA-damage regulated autophagy modulator 2
Target Gene
DRAM2
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ISLRVEANLHGLTLYDTAPCPINNERTRLLSRDI
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000027900 (85%)

Rat ENSRNOG00000017744 (82%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
DNA-damage regulated autophagy modulator 2
Gene Name
DRAM2
Alternative Gene Names
MGC54289, PRO180, RP5-1180E21.1, TMEM77, WWFQ154
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000156171 on the Human Protein Atlas

Li Q, Fang Y, Zhu P, Ren CY, Chen H, Gu J, Jia YP, Wang K, Tong WD, Zhang WJ, Pan J, Lu DS, Tang B, Mao XH

Burkholderia pseudomallei survival in lung epithelial cells benefits from miRNA-mediated suppression of ATG10

Autophagy , 2015 Jul 7; 11(8):1293-1307. Epub 2015 Sep 14

PubMed ID: 26151773 DOI: 10.1080/15548627.2015.1058474

Zeng CW, Chen ZH, Zhang XJ, Han BW, Lin KY, Li XJ, Wei PP, Zhang H, Li Y, Chen YQ

MIR125B1 represses the degradation of the PML-RARA oncoprotein by an autophagy-lysosomal pathway in acute promyelocytic leukemia

Autophagy , 2014 Oct 1; 10(10):1726-1737. Epub 2014 Jul 18

PubMed ID: 25126724 DOI: 10.4161/auto.29592

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Alternative Antibodies
Corresponding Antigens

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