Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Contribution from The Human Protein Atlas (proteinatlas.org) — 2015-10-16 00:00:00
The Human Protein Atlas has successfully tested the Anti-DNTTIP2 antibody (HPA044502) in siRNA transfected U-2 OS cells
A. To validate the subcellular localization of the Anti-DNTTIP2 antibody (HPA044502), determined by immunofluorescence, the staining procedure was repeated on siRNA transfected U-2 OS cells. The assay was performed in using siRNA from one provider (siRNA 1) and the results were compared to negative control cells transfected with scrambled siRNA.
Negative control (scrambled) siRNA transfected U-2 OS cells
The images show signal downregulation > 25% compared to the negative control (scrambled). Relative Fluorescence Intensity (RFI) denotes the percentage of remaining staining intensity after siRNA down regulation.
B. The Anti-DNTTIP2 antibody (HPA044502) was tested in Western blot using siRNA transfected U-2 OS cells, from two different providers (siRNA 1 and siRNA 2), and the result shows downregulation in both siRNA lanes compared to the control (scrambled).
Western blot image for the HPA044502 antibody using lysates from siRNA transfected U-2 OS cells.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: scrambled
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