Polyclonal Anti-DNPEP Antibody
Lane 2: Human cell line RT-4
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000019772 (91%)
Mouse ENSMUSG00000026209 (90%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-DNPEP HPA044860.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria
PLoS Pathog , 2014 Apr; 10(4):e1004038. Epub 2014 Apr 17
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