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Enhanced Validation

Anti-DNMT3B Antibody

DNA (cytosine-5-)-methyltransferase 3 beta
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human DNMT3B
Open Datasheet
Price
$450.00
Product Number
HPA001595
Unit Size
Concentration
Lot dependent
Availability
7 in Stock
Ships
Ready to Ship
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[3 pictures]
Immunohistochemistry analysis in human testis and skeletal muscle tissues using Anti-DNMT3B antibody. Corresponding DNMT3B RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in human cell lines Caco-2 and SK-MEL-30 using Anti-DNMT3B antibody. Corresponding DNMT3B RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
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[1 pictures]
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
DNA (cytosine-5-)-methyltransferase 3 beta
Target Gene
DNMT3B
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
MEPSPEPPSLESMKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHVDES
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000010625 (66%)

Mouse ENSMUSG00000027478 (62%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA001595 on the Human Protein Atlas
Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA001595 on the Human Protein Atlas
Protein Name
DNA (cytosine-5-)-methyltransferase 3 beta
Gene Name
DNMT3B
UniProt ID
Q9UBC3
Gene (Ensembl)
ENSG00000088305
Entrez Gene ID
Entrez gene: 1789
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000088305 on the Human Protein Atlas

Xie Z, Dang Y, Wu H, He R, Ma J, Peng Z, Rong M, Li Z, Yang J, Jiang Y, Chen G, Yang L

Effect of CELSR3 on the Cell Cycle and Apoptosis of Hepatocellular Carcinoma Cells

J Cancer , 2020 Feb 21; 11(10):2830-2844. Epub 2020 Feb 21

PubMed ID: 32226501 DOI: 10.7150/jca.39328

Ferreira HJ, Davalos V, de Moura MC, Soler M, Perez-Salvia M, Bueno-Costa A, Setien F, Moran S, Villanueva A, Esteller M

Circular RNA CpG island hypermethylation-associated silencing in human cancer

Oncotarget , 2018 Jun 26; 9(49):29208-29219. Epub 2018 Jun 26

PubMed ID: 30018746 DOI: 10.18632/oncotarget.25673

Chomyk AM, Volsko C, Tripathi A, Deckard SA, Trapp BD, Fox RJ, Dutta R

DNA methylation in demyelinated multiple sclerosis hippocampus

Sci Rep , 2017 Aug 18; 7:8696. Epub 2017 Aug 18

PubMed ID: 28821749 DOI: 10.1038/s41598-017-08623-5

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Corresponding Antigens
PrEST Antigen DNMT3B (APrEST78217)

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