Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000010625 (66%)
Mouse ENSMUSG00000027478 (62%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Effect of CELSR3 on the Cell Cycle and Apoptosis of Hepatocellular Carcinoma Cells
J Cancer , 2020 Feb 21; 11(10):2830-2844. Epub 2020 Feb 21
Circular RNA CpG island hypermethylation-associated silencing in human cancer
Oncotarget , 2018 Jun 26; 9(49):29208-29219. Epub 2018 Jun 26
DNA methylation in demyelinated multiple sclerosis hippocampus
Sci Rep , 2017 Aug 18; 7:8696. Epub 2017 Aug 18
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