Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000034480 (82%)
Rat ENSRNOG00000019688 (39%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Generation of stress fibers through myosin-driven reorganization of the actin cortex
eLife , 2021 Jan 28; 10:e60710. Epub 2021 Jan 28
The formin Drosophila homologue of Diaphanous2 (Diaph2) controls microtubule dynamics in colorectal cancer cells independent of its FH2-domain
Sci Rep , 2019 Mar 29; 9:5352. Epub 2019 Mar 29
A role for mDia, a Rho-regulated actin nucleator, in tangential migration of interneuron precursors.
Nat Neurosci , 2012 Jan 15; 15(3):373-80, S1-2. Epub 2012 Jan 15
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