Polyclonal Anti-DES Antibody
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000019810 (98%)
Mouse ENSMUSG00000026208 (98%)
Recommended conditions:
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000175084 on the Human Protein Atlas
Tissue specific protein expression in human cells, tissues and organs
J Proteomics Bioinform , September 24, 2010; 3:286- 293
Inhibition of ROCK1 kinase modulates both tumor cells and stromal fibroblasts in pancreatic cancer
PLOS ONE , August 25, 2017
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