Polyclonal Anti-DDX3X Antibody



Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000023383 (97%)
Mouse ENSMUSG00000000787 (97%)
Recommended conditions:
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000215301 on the Human Protein Atlas
C9orf72 arginine-rich dipeptide repeat proteins disrupt karyopherin-mediated nuclear import
eLife , 2020 Mar 2; 9:e51685. Epub 2020 Mar 2
Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS
Biochim Biophys Acta , 2016 Jul 25; 1862(10):2004-2014. Epub 2016 Jul 25
Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer drivers
Cancer Discov , 2013 Sep; 3(9):1044-1057. Epub 2013 Jun 13
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics
Mol Cell Proteomics , 2009 Jul; 8(7):1612-1622. Epub 2009 Apr 7
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