Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000023474 (92%)
Mouse ENSMUSG00000010592 (92%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-DAZL HPA019593.
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
The human testis-specific proteome defined by transcriptomics and antibody-based profiling.
Mol Hum Reprod , 2014 Jun; 20(6):476-88. Epub 2014 Mar 5
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