Enhanced Validation

Anti-CYP24A1 Antibody

cytochrome P450, family 24, subfamily A, polypeptide 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human CYP24A1
Alternative Gene Names
CP24, CYP24, P450-CC24
Price
$445.00
Product Number
HPA022261
Unit Size
Concentration
Lot dependent
Availability
On Demand
Ships
On Demand
Target Protein
cytochrome P450, family 24, subfamily A, polypeptide 1
Target Gene
CYP24A1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQV
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000038567 (91%)

Rat ENSRNOG00000013062 (90%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
cytochrome P450, family 24, subfamily A, polypeptide 1
Gene Name
CYP24A1
Alternative Gene Names
CP24, CYP24, P450-CC24
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000019186 on the Human Protein Atlas

Lund RJ, Huhtinen K, Salmi J, Rantala J, Nguyen EV, Moulder R, Goodlett DR, Lahesmaa R, Carpén O

DNA methylation and Transcriptome Changes Associated with Cisplatin Resistance in Ovarian Cancer

Sci Rep , 2017 May 4; 7:1469. Epub 2017 May 4

PubMed ID: 28473707 DOI: 10.1038/s41598-017-01624-4

Smolders J, Schuurman KG, van Strien ME, Melief J, Hendrickx D, Hol EM, van Eden C, Luchetti S, Huitinga I

Expression of vitamin D receptor and metabolizing enzymes in multiple sclerosis-affected brain tissue.

J Neuropathol Exp Neurol , 2013 Feb; 72(2):91-105

PubMed ID: 23334593 DOI: 10.1097/NEN.0b013e31827f4fcc

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Alternative Antibodies
Corresponding Antigens

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