Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000024365 (69%)
Rat ENSRNOG00000000428 (67%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-CYP21A2 HPA053371.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
The human fetal adrenal produces cortisol but no detectable aldosterone throughout the second trimester
BMC Med , 2018 Feb 12; 16:23. Epub 2018 Feb 12
Adrenal Development in Mice Requires GATA4 and GATA6 Transcription Factors
Endocrinology , 2015 May 1; 156(7):2503-2517. Epub 2015 May 1
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