Enhanced Validation

Anti-COL3A1 Antibody

collagen, type III, alpha 1
Recommended Applications
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human COL3A1
Alternative Gene Names
Product Number
Unit Size
100 µl
Lot dependent
5 in Stock
Ready to Ship
Target Protein
collagen, type III, alpha 1
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000026043 (93%)

Rat ENSRNOG00000003357 (91%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
collagen, type III, alpha 1
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000168542 on the Human Protein Atlas

Davaadelger B, Choi MR, Singhal H, Clare SE, Khan SA, Kim JJ

BRCA1 mutation influences progesterone response in human benign mammary organoids

Breast Cancer Res , 2019 Nov 26; 21:124. Epub 2019 Nov 26

PubMed ID: 31771627 DOI: 10.1186/s13058-019-1214-0

Gao YF, Zhu T, Chen J, Liu L, Ouyang R

Knockdown of collagen α-1(III) inhibits glioma cell proliferation and migration and is regulated by miR128-3p

Oncol Lett , 2018 May 30; 16(2):1917-1923. Epub 2018 May 30

PubMed ID: 30008884 DOI: 10.3892/ol.2018.8830

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Corresponding Antigens


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