Enhanced Validation

Polyclonal Anti-CNP Antibody

2',3'-cyclic nucleotide 3' phosphodiesterase
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human CNP
Price
$459.00
Product Number
HPA023266
Unit Size
100 µl
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
2',3'-cyclic nucleotide 3' phosphodiesterase
Target Gene
CNP
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFG
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000017496 (90%)

Mouse ENSMUSG00000006782 (90%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
2',3'-cyclic nucleotide 3' phosphodiesterase
Gene Name
CNP
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000173786 on the Human Protein Atlas

Wüthrich C, Batson S, Anderson MP, White LR, Koralnik IJ

JC Virus Infects Neurons and Glial Cells in the Hippocampus

J Neuropathol Exp Neurol , 2016 Jun 12; 75(8):712-717. Epub 2016 Jun 12

PubMed ID: 27297673 DOI: 10.1093/jnen/nlw050

Peluffo H, Alí‐Ruiz D, Ejarque‐Ortíz A, Heras‐Alvarez V, Comas‐Casellas E, Martínez‐Barriocanal A, Kamaid A, Alvarez‐Errico D, Negro ML, Lago N, Schwartz Jr S, Villaverde A, Sayós J

Overexpression of the Immunoreceptor CD300f Has a Neuroprotective Role in a Model of Acute Brain Injury

Brain Pathol , 2011 Oct 31; 22(3):318-328. Epub 2011 Oct 31

PubMed ID: 21951326 DOI: 10.1111/j.1750-3639.2011.00537.x

Did we miss your publication?

Have you published using HPA023266? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch