Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000037242 (98%)
Rat ENSRNOG00000018109 (97%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-CLIC4 HPA060804.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Histamine H3 receptors aggravate cerebral ischaemic injury by histamine-independent mechanisms
Nat Commun , 2014 Feb 25; 5:3334. Epub 2014 Feb 25
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
Impact of genomic stability on protein expression in endometrioid endometrial cancer
Br J Cancer , 2012 Mar 27; 106(7):1297-1305. Epub 2012 Mar 13
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