Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000016234 (92%)
Mouse ENSMUSG00000031781 (89%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation
PLoS One , 2018 Mar 29; 13(3):e0194782. Epub 2018 Mar 29
The Diabetes Drug Target MitoNEET Governs a Novel Trafficking Pathway to Rebuild an Fe-S Cluster into Cytosolic Aconitase/Iron Regulatory Protein 1
J Biol Chem , 2014 Oct 10; 289(41):28070-28086. Epub 2014 Jul 10
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