Enhanced Validation

Anti-CHGA Antibody

chromogranin A (parathyroid secretory protein 1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
Product Description
Polyclonal Antibody against Human CHGA
Product Number
Unit Size
100 µl
Lot dependent
10 in Stock
Ready to Ship
Target Protein
chromogranin A (parathyroid secretory protein 1)
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000052549 (64%)

Mouse ENSMUSG00000021194 (62%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Recombinant expression validation in WB using target protein overexpression.

Protein Name
chromogranin A (parathyroid secretory protein 1)
Gene Name
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000100604 on the Human Protein Atlas

Chen HJ, Poran A, Unni AM, Huang SX, Elemento O, Snoeck HW, Varmus H

Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells

J Exp Med , 2019 Mar 4; 216(3):674-687

PubMed ID: 30737256 DOI: 10.1084/jem.20181155

Zhang D, Babayan L, Ho H, Heaney AP

Chromogranin A regulates neuroblastoma proliferation and phenotype

Biol Open , 2019 Mar 15; 8(3):bio036566. Epub 2019 Mar 15

PubMed ID: 30833285 DOI: 10.1242/bio.036566

Chen HJ, Wei Z, Sun J, Bhattacharya A, Savage DJ, Serda R, Mackeyev Y, Curley SA, Bu P, Wang L, Chen S, Cohen-Gould L, Huang E, Shen X, Lipkin SM, Copeland NG, Jenkins NA, Shuler ML

A recellularized human colon model identifies cancer driver genes

Nat Biotechnol , 2016 Jul 11; 34(8):845-851. Epub 2016 Jul 11

PubMed ID: 27398792 DOI: 10.1038/nbt.3586

Marbiah MM, Harvey A, West BT, Louzolo A, Banerjee P, Alden J, Grigoriadis A, Hummerich H, Kan HM, Cai Y, Bloom GS, Jat P, Collinge J, Klöhn PC

Identification of a gene regulatory network associated with prion replication

EMBO J , 2014 Jul 17; 33(14):1527-1547. Epub 2014 May 19

PubMed ID: 24843046 DOI: 10.15252/embj.201387150

Kiflemariam S, Andersson S, Asplund A, Pontén F, Sjöblom T

Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.

PLoS One , 2012; 7(3):e32927. Epub 2012 Mar 8

PubMed ID: 22412953 DOI: 10.1371/journal.pone.0032927

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