Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells
J Exp Med , 2019 Mar 4; 216(3):674-687
Chromogranin A regulates neuroblastoma proliferation and phenotype
Biol Open , 2019 Mar 15; 8(3):bio036566. Epub 2019 Mar 15
A recellularized human colon model identifies cancer driver genes
Nat Biotechnol , 2016 Jul 11; 34(8):845-851. Epub 2016 Jul 11
Identification of a gene regulatory network associated with prion replication
EMBO J , 2014 Jul 17; 33(14):1527-1547. Epub 2014 May 19
Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.
PLoS One , 2012; 7(3):e32927. Epub 2012 Mar 8
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