Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000050097 (66%)
Rat ENSRNOG00000039079 (65%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Measuring human carboxylesterase 2 activity in pancreatic cancer patient-derived xenografts using a ratiometric fluorescent chemosensor †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sc00283a
Chem Sci , 2019 Jul 29; 10(36):8428-8437. Epub 2019 Jul 29
Prognostic and Functional Significance of MAP4K5 in Pancreatic Cancer
PLoS One , 2016 Mar 29; 11(3):e0152300. Epub 2016 Mar 29
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
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