Polyclonal Anti-CEP97 Antibody
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000001609 (68%)
Mouse ENSMUSG00000022604 (63%)
Recommended conditions:
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000182504 on the Human Protein Atlas
miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.
Nat Cell Biol , 2012 Jun 10; 14(7):697-706. Epub 2012 Jun 10
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