Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000037664 (76%)
Rat ENSRNOG00000059500 (76%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Abundances of placental imprinted genes CDKN1C, PHLDA2 and IGF-2 are related to low birth weight and early catch-up growth in full-term infants born small for gestational age
PLoS One , 2019 Jun 13; 14(6):e0218278. Epub 2019 Jun 13
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