Enhanced Validation

Anti-CD99 Antibody

CD99 molecule
Recommended Applications
Recombinant expression validation in WB using target protein overexpression.
Product Description
Polyclonal Antibody against Human CD99
Alternative Gene Names
MIC2
Price
$437.00
Product Number
HPA035304
Unit Size
100 µl
Concentration
Lot dependent
Availability
8 in Stock
Ships
Ready to Ship
Target protein
CD99 molecule
Target gene
CD99
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
SSFIAYQKKKLCFKENAEQGEVDMESHRNANAEPAVQRTLL
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000007090 (34%)

Mouse ENSMUSG00000051331 (34%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Concentration
Lot dependent
Unit Size
100 µl
Concentration
Lot dependent
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.4 µg/ml

Protocols
Western Blot (WB)

Recombinant expression validation in WB using target protein overexpression.

Protein Name
CD99 molecule
Gene Name
CD99
Alternative Gene Names
MIC2
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000002586 on the Human Protein Atlas

Yang B, Yang C, Fang J, Yang J, Xu Y

Clinicoradiological characteristics, management and prognosis of primary myeloid sarcoma of the central nervous system: A report of four cases

Oncol Lett , 2017 Jul 20; 14(3):3825-3831. Epub 2017 Jul 20

PubMed ID: 28927153 DOI: 10.3892/ol.2017.6620

Edlund K, Lindskog C, Saito A, Berglund A, Pontén F, Göransson-Kultima H, Isaksson A, Jirström K, Planck M, Johansson L, Lambe M, Holmberg L, Nyberg F, Ekman S, Bergqvist M, Landelius P, Lamberg K, Botling J, Ostman A, Micke P

CD99 is a novel prognostic stromal marker in non-small cell lung cancer.

Int J Cancer , 2012 Nov 15; 131(10):2264-73. Epub 2012 Apr 24

PubMed ID: 22392539 DOI: 10.1002/ijc.27518

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not articipate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Corresponding Antigens

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