Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000042821 (44%)
Mouse ENSMUSG00000068686 (42%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Pancreatic cancer-educated macrophages protect cancer cells from complement-dependent cytotoxicity by up-regulation of CD59
Cell Death Dis , 2019 Nov 4; 10(11):836. Epub 2019 Nov 4
Complement regulatory proteins in kidneys of patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis
Clin Exp Immunol , 2017 Oct 20; 191(1):116-124. Epub 2017 Oct 20
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