Enhanced Validation

Anti-CD44 Antibody

CD44 molecule (Indian blood group)
References (19)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human CD44
Alternative Gene Names
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Open Datasheet
Price
$450.00
Product Number
HPA005785
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human skin and skeletal muscle tissues using HPA005785 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in human cell lines U-251MG and MCF-7 using Anti-CD44 antibody. Corresponding CD44 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
CD44 molecule (Indian blood group)
Target Gene
CD44
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPR
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000005087 (51%)

Rat ENSRNOG00000006094 (47%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA005785 on the Human Protein Atlas
Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA005785 on the Human Protein Atlas
Protein Name
CD44 molecule (Indian blood group)
Gene Name
CD44
Alternative Gene Names
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
UniProt ID
P16070
Gene (Ensembl)
ENSG00000026508
Entrez Gene ID
Entrez gene: 960
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000026508 on the Human Protein Atlas

Li Y, Hu H, Wang Y, Fan Y, Yang Y, Guo B, Xie X, Lian J, Jiang B, Han B, Wang Y, Shao C, Gong Y

CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer

Oncogenesis , 2020 Feb 13; 9(2):20. Epub 2020 Feb 13

PubMed ID: 32054830 DOI: 10.1038/s41389-020-0206-3

Tsugawa H, Kato C, Mori H, Matsuzaki J, Kameyama K, Saya H, Hatakeyama M, Suematsu M, Suzuki H

Cancer Stem-Cell Marker CD44v9-Positive Cells Arise From Helicobacter pylori–Infected CAPZA1-Overexpressing Cells

Cell Mol Gastroenterol Hepatol , 2019 May 27; 8(3):319-334. Epub 2019 May 27

PubMed ID: 31146068 DOI: 10.1016/j.jcmgh.2019.05.008

Colin DJ, Cottet-Dumoulin D, Faivre A, Germain S, Triponez F, Serre-Beinier V

Experimental Model of Human Malignant Mesothelioma in Athymic Mice

Int J Mol Sci , 2018 Jun 26; 19(7):1881. Epub 2018 Jun 26

PubMed ID: 29949929 DOI: 10.3390/ijms19071881

Zhou D, Alver BM, Li S, Hlady RA, Thompson JJ, Schroeder MA, Lee JH, Qiu J, Schwartz PH, Sarkaria JN, Robertson KD

Distinctive epigenomes characterize glioma stem cells and their response to differentiation cues

Genome Biol , 2018 Mar 27; 19:43. Epub 2018 Mar 27

PubMed ID: 29587824 DOI: 10.1186/s13059-018-1420-6

Walker R, Poleszczuk J, Mejia J, Lee JK, Pimiento JM, Malafa M, Giuliano AR, Enderling H, Coppola D

Toward early detection of Helicobacter pylori-associated gastric cancer

Gastric Cancer , 2017 Jul 19; 21(2):196-203. Epub 2017 Jul 19

PubMed ID: 28725964 DOI: 10.1007/s10120-017-0748-z

Rho JH, Ladd JJ, Li CI, Potter JD, Zhang Y, Shelley D, Shibata D, Coppola D, Yamada H, Toyoda H, Tada T, Kumada T, Brenner DE, Hanash SM, Lampe PD

Protein and glycomic plasma markers for early detection of adenoma and colon cancer

Gut , 2016 Nov 7; 67(3):473-484. Epub 2016 Nov 7

PubMed ID: 27821646 DOI: 10.1136/gutjnl-2016-312794

den Uil SH, Coupé VM, Linnekamp JF, van den Broek E, Goos JA, Delis-van Diemen PM, Belt EJ, van Grieken NC, Scott PM, Vermeulen L, Medema JP, Bril H, Stockmann HB, Cormier RT, Meijer GA, Fijneman RJ

Loss of KCNQ1 expression in stage II and stage III colon cancer is a strong prognostic factor for disease recurrence

Br J Cancer , 2016 Nov 17; 115(12):1565-1574. Epub 2016 Nov 17

PubMed ID: 27855440 DOI: 10.1038/bjc.2016.376

Pokharel D, Padula MP, Lu JF, Jaiswal R, Djordjevic SP, Bebawy M

The Role of CD44 and ERM Proteins in Expression and Functionality of P-glycoprotein in Breast Cancer Cells

Molecules , 2016 Mar 1; 21(3):290. Epub 2016 Mar 1

PubMed ID: 26938523 DOI: 10.3390/molecules21030290

Monaghan MG, Linneweh M, Liebscher S, Van Handel B, Layland SL, Schenke-Layland K

Endocardial-to-mesenchymal transformation and mesenchymal cell colonization at the onset of human cardiac valve development

Development , 2016/02/01; 143(3):473-482

PubMed ID: 26674310 DOI: 10.1242/dev.133843

D'Amato-Brito C, Cipriano D, Colin DJ, Germain S, Seimbille Y, Robert JH, Triponez F, Serre-Beinier V

Role of MIF/CD74 signaling pathway in the development of pleural mesothelioma

Oncotarget , 2016 Feb 11; 7(10):11512-11525. Epub 2016 Feb 11

PubMed ID: 26883190 DOI: 10.18632/oncotarget.7314

Lei J, Firdaus W, Rosenzweig JM, Alrebh S, Bakhshwin A, Borbiev T, Fatemi A, Blakemore K, Johnston MV, Burd I

Murine model: maternal administration of stem cells for prevention of prematurity

Am J Obstet Gynecol , 2014 Dec 30; 212(5):639.e1-639.10. Epub 2014 Dec 30

PubMed ID: 25555657 DOI: 10.1016/j.ajog.2014.12.032

Ran Ma, Irma Fredriksson, Govindasamy-Muralidharan Karthik, Gregory Winn, Eva Darai-Ramqvist, Jonas Bergh, Johan Hartman

Superficial scrapings from breast tumors is a source for biobanking and research purposes

Laboratory Investigation , 94, 796-805 (2014)

PubMed ID: 0 DOI: 10.1038/labinvest.2014.65

Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bäuerle T, Wallwiener M, Holland-Letz T, Höfner T, Sprick M, Scharpff M, Marmé F, Sinn HP, Pantel K, Weichert W, Trumpp A

Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.

Nat Biotechnol , 2013 Jun; 31(6):539-44. Epub 2013 Apr 21

PubMed ID: 23609047 DOI: 10.1038/nbt.2576

Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Némati F, Laval J, Château-Joubert S, Servely JL, Vallerand D, Fontaine JJ, Taylor N, Battini JL, Sitbon M, Decaudin D

Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters.

Lab Invest , 2013 May; 93(5):611-21. Epub 2013 Mar 4

PubMed ID: 23459372 DOI: 10.1038/labinvest.2013.44

Vazquez-Martin A, Oliveras-Ferraros C, Cufí S, Del Barco S, Martin-Castillo B, Menendez JA

Metformin regulates breast cancer stem cell ontogeny by transcriptional regulation of the epithelial-mesenchymal transition (EMT) status.

Cell Cycle , 2010 Sep 15; 9(18):3807-14. Epub 2010 Sep 25

PubMed ID: 20890129

Twarock S, Röck K, Sarbia M, Weber A, Jänicke R, Fischer J

Synthesis of hyaluronan in oesophageal cancer cells is uncoupled from the prostaglandin–cAMP pathway

Br J Pharmacol , 2009 May; 157(2):234-243. Epub 2009 Mar 26

PubMed ID: 19338584 DOI: 10.1111/j.1476-5381.2009.00138.x

Asplund A, Gry Björklund M, Sundquist C, Strömberg S, Edlund K, Ostman A, Nilsson P, Pontén F, Lundeberg J

Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma.

Br J Dermatol , 2008 Mar; 158(3):527-38. Epub 2008 Jan 30

PubMed ID: 18241271 DOI: 10.1111/j.1365-2133.2007.08418.x

Schneck H, Gierke B, Uppenkamp F, Behrens B, Niederacher D, Stoecklein NH, Templin MF, Pawlak M, Fehm T, Neubauer H, Disseminated Cancer Cell Network (DCC Net) Duesseldorf

EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer

PLoS One , 1/01/01; 10(12):e0144535. Epub 2015 Dec 22

PubMed ID: 26695635 DOI: 10.1371/journal.pone.0144535

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Corresponding Antigens
PrEST Antigen CD44 (APrEST83079)

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