Enhanced Validation

Polyclonal Anti-CD4 Antibody

CD4 molecule
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human CD4
Product Number
Unit Size
100 µl
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
CD4 molecule
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000016294 (57%)

Mouse ENSMUSG00000023274 (55%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
CD4 molecule
Gene Name
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000010610 on the Human Protein Atlas

Chinnapaiyan S, Dutta R, Bala J, Parira T, Agudelo M, Nair M, Unwalla HJ

Cigarette smoke promotes HIV infection of primary bronchial epithelium and additively suppresses CFTR function

Sci Rep , 2018 May 22; 8:7984. Epub 2018 May 22

PubMed ID: 29789655 DOI: 10.1038/s41598-018-26095-z

Yamada D, Ishimoto H, Li X, Kohashi T, Ishikawa Y, Kamikouchi A

GABAergic Local Interneurons Shape Female Fruit Fly Response to Mating Songs

J Neurosci , 2018 May 2; 38(18):4329-4347

PubMed ID: 29691331 DOI: 10.1523/JNEUROSCI.3644-17.2018

Gondim MV, Wiltzer-Bach L, Maurer B, Banning C, Arganaraz E, Schindler M

AP-2 Is the Crucial Clathrin Adaptor Protein for CD4 Downmodulation by HIV-1 Nef in Infected Primary CD4+ T Cells

J Virol , 2015 Sep 30; 89(24):12518-12524. Epub 2015 Sep 30

PubMed ID: 26423947 DOI: 10.1128/JVI.01838-15

Miyazaki T, Lin TY, Ito K, Lee CH, Stopfer M

A gustatory second-order neuron that connects sucrose-sensitive primary neurons and a distinct region of the gnathal ganglion in the Drosophila brain

J Neurogenet , 2015/01/01; 29(0):144-155. Epub 2015 Aug 27

PubMed ID: 26004543 DOI: 10.3109/01677063.2015.1054993

Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM

Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

Proteome Sci , 2011 Nov 17; 9:73. Epub 2011 Nov 17

PubMed ID: 22093360 DOI: 10.1186/1477-5956-9-73

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Corresponding Antigens


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