Polyclonal Anti-CD276 Antibody
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000035914 (89%)
Rat ENSRNOG00000033608 (89%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000103855 on the Human Protein Atlas
Large-scale analysis reveals the specific clinical and immune features of B7-H3 in glioma
Oncoimmunology , 2018 Aug 23; 7(11):e1461304. Epub 2018 Aug 23
Costimulatory protein 4IgB7H3 drives the malignant phenotype of glioblastoma by mediating immune escape and invasiveness.
Clin Cancer Res , 2012 Jan 1; 18(1):105-17. Epub 2011 Nov 11
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Contribution from Undisclosed customer — April 26, 2018
Western blot using Anti-CD276 (B7-H3) antibody (HPA017139) in U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line.
Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line.
PVDF membrane was probed with 0.5 ug/mL of Anti-CD276 (B7-H3) antibody (HPA017139). A specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, whereas in the knockout U2OS cell line no band was detected
The experiment was conducted under reducing conditions.
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