Enhanced Validation

Polyclonal Anti-CD276 Antibody

CD276 molecule
References (2) Researcher contributions (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
Product Description
Polyclonal Antibody against Human CD276
Alternative Gene Names
B7-H3, B7H3, B7RP-2
Open Datasheet
Price
$505.00
Product Number
HPA009285
Unit Size
Concentration
Lot dependent
Availability
8 in Stock
Ships
Ready to Ship
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[3 pictures]
Immunohistochemistry analysis in human prostate and pancreas tissues using Anti-CD276 antibody. Corresponding CD276 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410803).

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
CD276 molecule
Target Gene
CD276
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
YSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000035914 (95%)

Rat ENSRNOG00000033608 (95%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA009285 on the Human Protein Atlas
Western Blot (WB)

Recombinant expression validation in WB using target protein overexpression.

Protein Name
CD276 molecule
Gene Name
CD276
Alternative Gene Names
B7-H3, B7H3, B7RP-2
UniProt ID
Q5ZPR3
Gene (Ensembl)
ENSG00000103855
Entrez Gene ID
Entrez gene: 80381
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000103855 on the Human Protein Atlas

Chen L, Huang X, Zhang W, Liu Y, Chen B, Xiang Y, Zhang R, Zhang M, Feng J, Liu S, Duan T, Chen X, Wang W, Pan T, Yan L, Jin T, Li G, Li Y, Xie T, Sui X

Correlation of PD-L1 and SOCS3 Co-expression with the Prognosis of Hepatocellular Carcinoma Patients

J Cancer , 2020 Jul 11; 11(18):5440-5448. Epub 2020 Jul 11

PubMed ID: 32742491 DOI: 10.7150/jca.46158

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User Data, Western Blot KO, Customer Submission

Contribution from Undisclosed customer — April 26, 2018

Western blot using Anti-CD276 (B7-H3) antibody (HPA009285) in U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line.

 amab90516_wb_ko

Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line.

PVDF membrane was probed with 0.5 ug/mL of Anti-CD276 (B7-H3) antibody (HPA009285). A specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, whereas in the knockout U2OS cell line no band was detected

The experiment was conducted under reducing conditions.

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-CD276 (HPA017139)
Corresponding Antigens
PrEST Antigen CD276 (APrEST71718)

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