Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000051439 (65%)
Rat ENSRNOG00000017819 (63%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-CD14 HPA001887.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Epitope Recognition in the Human–Pig Comparison Model on Fixed and Embedded Material
J Histochem Cytochem , 2015 Jun 24; 63(10):805-822. Epub 2015 Jun 24
Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria
PLoS Pathog , 2014 Apr; 10(4):e1004038. Epub 2014 Apr 17
Candidate Serological Biomarkers for Cancer Identified from the Secretomes of 23 Cancer Cell Lines and the Human Protein Atlas
Mol Cell Proteomics , 2010 Jun; 9(6):1100-1117. Epub 2010 Feb 1
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