Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000051439 (57%)
Rat ENSRNOG00000017819 (55%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-CD14 HPA002127.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Identification of novel, immune-mediating extracellular vesicles in human lymphatic effluent draining primary cutaneous melanoma
Oncoimmunology , 2019 Sep 30; 8(12):e1667742. Epub 2019 Sep 30
Wingless ligand 5a is a critical regulator of placental growth and survival
Sci Rep , 2016 Jun 17; 6:28127. Epub 2016 Jun 17
Increased CTLA-4+ T cells and an increased ratio of monocytes with loss of class II (CD14+ HLA-DRlo/neg) found in aggressive pediatric sarcoma patients
J Immunother Cancer , 2015; 3:35. Epub 2015 Aug 18
LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14
Glia , 2014 Jun; 62(6):999-1013. Epub 2014 Mar 21
High-density lipoprotein endocytosis in endothelial cells
World J Biol Chem , 2013 Nov 26; 4(4):131-140. Epub 2013 Nov 26
Functional Contribution of Elevated Circulating and Hepatic Non-Classical CD14+CD16+ Monocytes to Inflammation and Human Liver Fibrosis
PLoS One , 2010 Jun 10; 5(6):e11049. Epub 2010 Jun 10
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