Enhanced Validation

Anti-CBLL1 Antibody

Cbl proto-oncogene-like 1, E3 ubiquitin protein ligase
Recommended Applications
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Product Description
Polyclonal Antibody against Human CBLL1
Alternative Gene Names
FLJ23109, HAKAI, RNF188
Price
$445.00
Product Number
HPA021773
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
Cbl proto-oncogene-like 1, E3 ubiquitin protein ligase
Target Gene
CBLL1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
RKHSNLITVPIQDDSNSGAREPPPPAPAPAHHHPEYQGQPVVSHPHHIMPPQQHYAPPPPPPPPISHPMP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000007253 (99%)

Mouse ENSMUSG00000020659 (99%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
Immunohistochemistry (IHC)

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-CBLL1 HPA026699.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
Cbl proto-oncogene-like 1, E3 ubiquitin protein ligase
Gene Name
CBLL1
Alternative Gene Names
FLJ23109, HAKAI, RNF188
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000105879 on the Human Protein Atlas

Hui L, Zhang S, Wudu M, Ren H, Xu Y, Zhang Q, Qiu X

CBLL1 is highly expressed in non‐small cell lung cancer and promotes cell proliferation and invasion

Thorac Cancer , 2019 May 23; 10(6):1479-1488. Epub 2019 May 23

PubMed ID: 31124298 DOI: 10.1111/1759-7714.13097

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Alternative Antibodies
Corresponding Antigens

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