Lane 2: Human cell line RT-4
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000053819 (100%)
Rat ENSRNOG00000018712 (100%)
Dilution: 1:20 - 1:50
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
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