Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-CALD1 HPA008066.
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Profiling post-centrifugation delay of serum and plasma with antibody bead arrays.
J Proteomics , 2013 Dec 16; 95:46-54. Epub 2013 Apr 28
Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection
J Histochem Cytochem , 2013 Nov; 61(11):773-784
Histochemical Localization of Caldesmon in the CNS and Ganglia of the Mouse
J Histochem Cytochem , 2011 May; 59(5):504-517
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