Polyclonal Anti-CALB2 Antibody
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000016977 (100%)
Mouse ENSMUSG00000003657 (100%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-CALB2 HPA007305.
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression
Front Oncol , 2018 Feb 23; 8:40. Epub 2018 Feb 23
Identification of cis- and trans-acting elements regulating calretinin expression in mesothelioma cells
Oncotarget , 2016 Feb 1; 7(16):21272-21286. Epub 2016 Feb 1
Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics
Mol Cell Proteomics , 2009 Jul; 8(7):1612-1622. Epub 2009 Apr 7
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.