Polyclonal Anti-BRAF Antibody

B-Raf proto-oncogene, serine/threonine kinase
References (1)
Recommended Applications
Product Description
Polyclonal Antibody against Human BRAF
Alternative Gene Names
BRAF1
Open Datasheet
Price
$505.00
Product Number
HPA071048
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[1 pictures]
Western blot analysis in human cell line BEWO.
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[1 pictures]
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.

Additional Images

See additional ICC images using this antibody on the Human Protein Atlas
Target Protein
B-Raf proto-oncogene, serine/threonine kinase
Target Gene
BRAF
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQL
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000002413 (100%)

Rat ENSRNOG00000010957 (100%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA071048 on the Human Protein Atlas
Protein Name
B-Raf proto-oncogene, serine/threonine kinase
Gene Name
BRAF
Alternative Gene Names
BRAF1
UniProt ID
P15056
Gene (Ensembl)
ENSG00000157764
Entrez Gene ID
Entrez gene: 673
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000157764 on the Human Protein Atlas

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Have you published using HPA071048? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-BRAF (AMAb91257)
Anti-BRAF (AMAb91258)
Anti-BRAF (HPA001328)
Corresponding Antigens
PrEST Antigen BRAF (APrEST90317)

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