Enhanced Validation

Anti-BMI1 Antibody

BMI1 proto-oncogene, polycomb ring finger
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human BMI1
Alternative Gene Names
PCGF4, RNF51
Price
$459.00
Product Number
HPA030472
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
BMI1 proto-oncogene, polycomb ring finger
Target Gene
BMI1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
RPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000016585 (95%)

Mouse ENSMUSG00000026739 (95%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
BMI1 proto-oncogene, polycomb ring finger
Gene Name
BMI1
Alternative Gene Names
PCGF4, RNF51
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000168283 on the Human Protein Atlas

Koren A, Rijavec M, Sodja E, Kern I, Sadikov A, Kovac V, Korosec P, Cufer T

High BMI1 mRNA expression in peripheral whole blood is associated with favorable prognosis in advanced non-small cell lung cancer patients

Oncotarget , 2017 Mar 6; 8(15):25384-25394. Epub 2017 Mar 6

PubMed ID: 28445986 DOI: 10.18632/oncotarget.15914

Kaufhold S, Garbán H, Bonavida B

Yin Yang 1 is associated with cancer stem cell transcription factors (SOX2, OCT4, BMI1) and clinical implication

J Exp Clin Cancer Res , 2016 May 25; 35:84. Epub 2016 May 25

PubMed ID: 27225481 DOI: 10.1186/s13046-016-0359-2

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Alternative Antibodies
Corresponding Antigens

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