Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000004446 (64%)
Rat ENSRNOG00000012439 (61%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Exploring the Contextual Sensitivity of Factors that Determine Cell-to-Cell Variability in Receptor-Mediated Apoptosis
PLoS Comput Biol , 2012; 8(4):e1002482. Epub 2012 Apr 26
Lyapunov exponents and phase diagrams reveal multi-factorial control over TRAIL-induced apoptosis
Mol Syst Biol , 2011 Nov 22; 7:553. Epub 2011 Nov 22
Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis.
Nature , 2009 May 21; 459(7245):428-32. Epub 2009 Apr 12
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