Enhanced Validation

Polyclonal Anti-B2M Antibody

beta-2-microglobulin
References (5)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human B2M
Open Datasheet
Price
$505.00
Product Number
HPA006361
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human lymph node and skeletal muscle tissues using HPA006361 antibody. Corresponding B2M RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in human cell lines HeLa and HEK293 using Anti-B2M antibody. Corresponding B2M RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
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[1 pictures]
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
beta-2-microglobulin
Target Gene
B2M
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000017123 (74%)

Mouse ENSMUSG00000060802 (69%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA006361 on the Human Protein Atlas
Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA006361 on the Human Protein Atlas
Protein Name
beta-2-microglobulin
Gene Name
B2M
UniProt ID
P61769
Gene (Ensembl)
ENSG00000166710
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000166710 on the Human Protein Atlas

Takeuchi M, Miyoshi H, Asano N, Yoshida N, Yamada K, Yanagida E, Moritsubo M, Nakata M, Umeno T, Suzuki T, Komaki S, Muta H, Furuta T, Seto M, Ohshima K

Human leukocyte antigen class II expression is a good prognostic factor in adult T-cell leukemia/lymphoma

Haematologica , 2019 Aug; 104(8):1626-1632

PubMed ID: 30630986 DOI: 10.3324/haematol.2018.205567

Andreas Blees, Dovile Januliene, Tommy Hofmann, Nicole Koller, Carla Schmidt, Simon Trowitzsch, Arne Moeller, Robert Tampé

Structure of the human MHC-I peptide-loading complex

Nature , 551, 525-528 (2017)

PubMed ID: None DOI: 10.1038/nature24627

Haworth KB, Arnold MA, Pierson CR, Choi K, Yeager ND, Ratner N, Roberts RD, Finlay JL, Cripe TP

Immune profiling of NF1-associated tumors reveals histologic subtype distinctions and heterogeneity: implications for immunotherapy

Oncotarget , 2017 May 30; 8(47):82037-82048. Epub 2017 May 30

PubMed ID: 29137242 DOI: 10.18632/oncotarget.18301

Lindskog C, Asplund A, Engkvist M, Uhlen M, Korsgren O, Ponten F

Antibody-based proteomics for discovery and exploration of proteins expressed in pancreatic islets.

Discov Med , 2010 Jun; 9(49):565-78

PubMed ID: 20587347

Did we miss your publication?

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens
PrEST Antigen B2M (APrEST71133)

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