Anti-ATRX Antibody



Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000056703 (97%)
Mouse ENSMUSG00000031229 (96%)
Recommended conditions:
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000085224 on the Human Protein Atlas
Generation of Glioblastoma Patient-Derived Intracranial Xenografts for Preclinical Studies
Int J Mol Sci , 2020 Jul 20; 21(14):5113. Epub 2020 Jul 20
Transformation Foci in IDH1-mutated Gliomas Show STAT3 Phosphorylation and Downregulate the Metabolic Enzyme ETNPPL, a Negative Regulator of Glioma Growth
Sci Rep , 2020 Mar 26; 10:5504. Epub 2020 Mar 26
Alternative lengthening of telomeres is the major telomere maintenance mechanism in astrocytoma with isocitrate dehydrogenase 1 mutation
J Neurooncol , 2020 Jan 20; 147(1):1-14. Epub 2020 Jan 20
Clinico-neuropathological features of isocitrate dehydrogenase 2 gene mutations in lower-grade gliomas
Chin Med J (Engl) , 2019 Dec 20; 132(24):2920-2926. Epub 2019 Dec 20
DNA Methylation Profiling Reveals Prognostically Significant Groups in Pediatric Adrenocortical Tumors: A Report From the International Pediatric Adrenocortical Tumor Registry
JCO Precis Oncol , 2019 Nov 18; 3:PO.19.00163. Epub 2019 Nov 18
ATRX in-frame fusion neuroblastoma is sensitive to EZH2 inhibition via modulation of neuronal gene signatures
Cancer Cell , 2019 Oct 17; 36(5):512-527.e9. Epub 2019 Oct 17
Telomere alterations in neurofibromatosis type 1-associated solid tumors
Acta Neuropathol Commun , 2019 Aug 28; 7:139. Epub 2019 Aug 28
Recurrent Glioma With Lineage Conversion From Oligodendroglioma to Astrocytoma in Two Cases
Front Oncol , 2019 Aug 27; 9:828. Epub 2019 Aug 27
ATRX Mutations in Pineal Parenchymal Tumors of Intermediate Differentiation
J Neuropathol Exp Neurol , 2019 May 27; 78(8):703-708. Epub 2019 May 27
Pituitary Adenoma in Pediatric and Adolescent Populations
J Neuropathol Exp Neurol , 2019 Apr 26; 78(7):626-632. Epub 2019 Apr 26
Rapid and reversible suppression of ALT by DAXX in osteosarcoma cells
Sci Rep , 2019 Mar 14; 9:4544. Epub 2019 Mar 14
Synthetic lethality of cytolytic HSV-1 in cancer cells with ATRX and PML deficiency
J Cell Sci , 2019 Mar 14; 132(5):jcs222349. Epub 2019 Mar 14
Undifferentiated Sarcomas Develop through Distinct Evolutionary Pathways
Cancer Cell , 2019 Mar 18; 35(3):441-456.e8
Grading of Neuroendocrine Carcinomas: Correlation of 68Ga-PET/CT Scan with Tissue Biomarkers
Dis Markers , 2018 Dec 2; 2018:6878409. Epub 2018 Dec 2
The molecular landscape of glioma in patients with Neurofibromatosis 1
Nat Med , 2018 Dec 10; 25(1):176-187. Epub 2018 Dec 10
Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
Croat Med J , 2018 Oct; 59(5):213-223
Significance of TERT and ATRX mutations in glioma
Oncol Lett , 2018 Oct 29; 17(1):95-102. Epub 2018 Oct 29
ALTERNATIVE LENGTHENING OF TELOMERES, ATRX LOSS AND H3-K27M MUTATIONS IN HISTOLOGICALLY DEFINED PILOCYTIC ASTROCYTOMA WITH ANAPLASIA
Brain Pathol , 2018 Oct 17; 29(1):126-140. Epub 2018 Oct 17
GRANULAR CELL ASTROCYTOMA: AN AGGRESSIVE IDH-WILDTYPE DIFFUSE GLIOMA WITH MOLECULAR GENETIC FEATURES OF PRIMARY GLIOBLASTOMA
Brain Pathol , 2018 Oct 10; 29(2):193-204. Epub 2018 Oct 10
ATRX loss induces multiple hallmarks of the alternative lengthening of telomeres (ALT) phenotype in human glioma cell lines in a cell line-specific manner
PLoS One , 2018 Sep 18; 13(9):e0204159. Epub 2018 Sep 18
Inhibition of GPR158 by microRNA-449a suppresses neural lineage of glioma stem/progenitor cells and correlates with higher glioma grades
Oncogene , 2018 May 3; 37(31):4313-4333. Epub 2018 May 3
The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma
Nat Commun , 2018 May 25; 9:2087. Epub 2018 May 25
Aberrant ATRX protein expression is associated with poor overall survival in NF1-MPNST
Oncotarget , 2018 May 1; 9(33):23018-23028. Epub 2018 May 1
Elevated TERT Expression in TERT-Wildtype Adult Diffuse Gliomas: Histological Evaluation with a Novel TERT-Specific Antibody
Biomed Res Int , 2018 Mar 5; 2018:7945845. Epub 2018 Mar 5
Microscopy with ultraviolet surface excitation for rapid slide-free histology
Nat Biomed Eng , 2017 Dec 4; 1:957-966. Epub 2017 Dec 4
Comparison among conventional and advanced MRI, 18F-FDG PET/CT, phenotype and genotype in glioblastoma
Oncotarget , 2017 Oct 4; 8(53):91636-91653. Epub 2017 Oct 4
Genomic analysis of the origins and evolution of multicentric diffuse lower-grade gliomas
Neuro Oncol , 2017 Oct 25; 20(5):632-641. Epub 2017 Oct 25
Primary Astrocytic Tumours and Paired Recurrences have Similar Biological Features in IDH1, TP53 and TERTp Mutation and MGMT, ATRX Loss
Sci Rep , 2017 Oct 12; 7:13038. Epub 2017 Oct 12
Reclassification of Mixed Oligoastrocytic Tumors Using a Genetically Integrated Diagnostic Approach
J Pathol Transl Med , 2017 Sep 29; 52(1):28-36. Epub 2017 Sep 29
The frequency and prognostic effect of TERT promoter mutation in diffuse gliomas
Acta Neuropathol Commun , 2017 Aug 29; 5:62. Epub 2017 Aug 29
Expression and prognostic value of JAM-A in gliomas
J Neurooncol , 2017 Jul 4; 135(1):107-117. Epub 2017 Jul 4
Expression of CD70 (CD27L) Is Associated With Epithelioid and Sarcomatous Features in IDH-Wild-Type Glioblastoma
J Neuropathol Exp Neurol , 2017 Jul 13; 76(8):697-708. Epub 2017 Jul 13
IDH1 or -2 mutations do not predict outcome and do not cause loss of 5-hydroxymethylcytosine or altered histone modifications in central chondrosarcomas
Clin Sarcoma Res , 2017 May 4; 7:8. Epub 2017 May 4
PARP1 expression and its correlation with survival is tumour molecular subtype dependent in glioblastoma
Oncotarget , 2017 May 19; 8(28):46348-46362. Epub 2017 May 19
DAXX/ATRX and MEN1 genes are strong prognostic markers in pancreatic neuroendocrine tumors
Oncotarget , 2017 May 18; 8(30):49796-49806. Epub 2017 May 18
Alternative lengthening of telomeres and ATRX/DAXX loss can reliably be detected in fine needle aspirates of pancreatic neuroendocrine tumors
Cancer , 2017 Apr 3; 125(7):544-551. Epub 2017 Apr 3
Somatostatin receptor 2A in gliomas: Association with oligodendrogliomas and favourable outcome
Oncotarget , 2017 Apr 13; 8(30):49123-49132. Epub 2017 Apr 13
Anti-proliferative and anti-secretory effects of everolimus on human pancreatic neuroendocrine tumors primary cultures: is there any benefit from combination with somatostatin analogs?
Oncotarget , 2017 Apr 10; 8(25):41044-41063. Epub 2017 Apr 10
P03.10 Combined immunohistochemistry for IDH1R132H, p53 and ATRX improves the molecular classification of diffuse gliomas in adults
Neuro Oncol , 2017 Apr 19; 19(Suppl 3):iii35. Epub 2017 Apr 19
Adult Infiltrating Gliomas with WHO 2016 Integrated Diagnosis: Additional Prognostic Roles of ATRX and TERT
Acta Neuropathol , 2017 Mar 2; 133(6):1001-1016. Epub 2017 Mar 2
Bithalamic Gliomas May Be Molecularly Distinct From Their Unilateral High-Grade Counterparts
Brain Pathol , 2017 Mar 12; 28(1):112-120. Epub 2017 Mar 12
Alternative lengthening of telomeres in primary pancreatic neuroendocrine neoplasms is associated with aggressive clinical behavior and poor survival
Clin Cancer Res , 2016 Sep 23; 23(6):1598-1606. Epub 2016 Sep 23
Cancer Cells with Alternative Lengthening of Telomeres Do Not Display a General Hypersensitivity to ATR Inhibition
Front Oncol , 2016 Aug 23; 6:186. Epub 2016 Aug 23
Not all 1p/19q non-codeleted oligodendroglial tumors are astrocytic
Oncotarget , 2016 Aug 18; 7(40):64615-64630. Epub 2016 Aug 18
TERT promoter mutations in pancreatic endocrine tumours are rare and mainly found in tumours from patients with hereditary syndromes
Sci Rep , 2016 Jul 14; 6:29714. Epub 2016 Jul 14
Alternative Lengthening of Telomeres and Loss of DAXX/ATRX Expression Predicts Metastatic Disease and Poor Survival in Patients with Pancreatic Neuroendocrine Tumors
Clin Cancer Res , 2016 Jul 12; 23(2):600-609. Epub 2016 Jul 12
IDH-mutant glioma specific association of rs55705857 located at 8q24.21 involves MYC deregulation
Sci Rep , 2016 Jun 10; 6:27569. Epub 2016 Jun 10
ATRX binds to atypical chromatin domains at the 3′ exons of zinc finger genes to preserve H3K9me3 enrichment
Epigenetics , 2016 Mar 30; 11(6):398-414. Epub 2016 Mar 30
Exome Sequencing of Uterine Leiomyosarcomas Identifies Frequent Mutations in TP53, ATRX, and MED12
PLoS Genet , 2016 Feb 18; 12(2):e1005850. Epub 2016 Feb 18
Genotyping low-grade gliomas among Hispanics
Neurooncol Pract , 2016 Jan 28; 3(3):164-172. Epub 2016 Jan 28
Diffuse Midline Gliomas with Histone H3-K27M Mutation: A Series of 47 Cases Assessing the Spectrum of Morphologic Variation and Associated Genetic Alterations
Brain Pathol , 2015 Dec 14; 26(5):569-580. Epub 2015 Dec 14
Gemcitabine Plus Radiation Therapy for High Grade Glioma: Long-term Results of a Phase I Dose-Escalation Study
Int J Radiat Oncol Biol Phys , 2015 Nov 10; 94(2):305-311. Epub 2015 Nov 10
TERT promoter mutations are frequent and show association with MED12 mutations in phyllodes tumors of the breast
British Journal of Cancer , September 10, 2015
Adult Diffuse Astrocytoma in the Medulla Oblongata: Molecular Biological Analyses Including H3F3A Mutation of Histone H3.3
NMC Case Rep J , 2015 Sep 8; 3(2):29-33. Epub 2015 Sep 8
Molecular–genetic and clinical characteristics of gliomas with astrocytic appearance and total 1p19q loss in a single institutional consecutive cohort
Oncotarget , 2015 Jun 30; 6(18):15871-15881. Epub 2015 May 11
ATRX represses alternative lengthening of telomeres
Oncotarget , 2015 Jun 30; 6(18):16543-16558. Epub 2015 Apr 15
CDKN2A Loss Is Associated with Shortened Overall Survival in Lower Grade (World Health Organization II-III) Astrocytomas
J Neuropathol Exp Neurol , 2015/05/01; 74(5):442-452
Loss of ATRX and DAXX expression identifies poor prognosis for smooth muscle tumours of uncertain malignant potential and early stage uterine leiomyosarcoma
J Pathol Clin Res , 2015 Mar 16; 1(2):95-105. Epub 2015 Mar 16
Whole-exome sequencing identifies somatic <named-entity>ATRX</named-entity> mutations in pheochromocytomas and paragangliomas
Nature Communications , 6, (2015)
Telomerase inhibition abolishes the tumorigenicity of pediatric ependymoma tumor-initiating cells
Acta Neuropathol , 2014 Dec; 128:863-877. Epub 2014 Aug 6
Clinical multiplexed exome sequencing distinguishes adult oligodendroglial neoplasms from astrocytic and mixed lineage gliomas
Oncotarget , 2014 Sep 30; 5(18):8083-8092. Epub 2014 Aug 12
Benefit From Procarbazine, Lomustine, and Vincristine in Oligodendroglial Tumors Is Associated With Mutation of IDH
J Clin Oncol , 2014 Mar 10; 32(8):783-790. Epub 2014 Feb 10
The alternative lengthening of telomere phenotype is significantly associated with loss of ATRX expression in high-grade pediatric and adult astrocytomas: a multi-institutional study of 214 astrocytomas.
Mod Pathol , 2013 Nov; 26(11):1425-32. Epub 2013 Jun 14
Molecular and morphologic correlates of the alternative lengthening of telomeres phenotype in high-grade astrocytomas.
Brain Pathol , 2013 May; 23(3):237-43. Epub 2012 Sep 24
Loss of ATRX or DAXX expression and concomitant acquisition of the alternative lengthening of telomeres phenotype are late events in a small subset of MEN-1 syndrome pancreatic neuroendocrine tumors
Mod Pathol , 2012 Jul; 25(7):1033-1039. Epub 2012 May 11
Frequent ATRX, CIC, FUBP1 and IDH1 mutations refine the classification of malignant gliomas
Oncotarget , 2012 Jul; 3(7):709-722. Epub 2012 Aug 3
Loss of ATRX, Genome Instability, and an Altered DNA Damage Response Are Hallmarks of the Alternative Lengthening of Telomeres Pathway
PLoS Genet , 2012; 8(7):e1002772. Epub 2012 Jul 19
Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma.
Nature , 2012 Jan 29; 482(7384):226-31. Epub 2012 Jan 29
Altered telomeres in tumors with ATRX and DAXX mutations.
Science , 2011 Jul 22; 333(6041):425. Epub 2011 Jun 30
Clinical Neuropathology practice news 2-2014: ATRX, a new candidate biomarker in gliomas
Clin Neuropathol , 2014 Mar-Apr; 33(2):108-111. Epub 2014 Mar 26
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