Enhanced Validation

Polyclonal Anti-ASRGL1 Antibody

asparaginase like 1
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
Product Description
Polyclonal Antibody against Human ASRGL1
Alternative Gene Names
ALP, ALP1, FLJ22316
Open Datasheet
Price
$505.00
Product Number
HPA029725
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[7 pictures]
Immunohistochemistry analysis in human fallopian tube and skeletal muscle tissues using HPA029725 antibody. Corresponding ASRGL1 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421255).
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[1 pictures]
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
asparaginase like 1
Target Gene
ASRGL1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
MDGKDLSAGAVSAVQCIANPIKLARLVMEKTPHCFLTDQGAAQFAAAMGVPEIPGEKLVTERNKKRLEKEKHEKGAQKTDCQ
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000020202 (79%)

Mouse ENSMUSG00000024654 (77%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-ASRGL1 HPA055572.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA029725 on the Human Protein Atlas
Western Blot (WB)

Recombinant expression validation in WB using target protein overexpression.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA029725 on the Human Protein Atlas
Protein Name
asparaginase like 1
Gene Name
ASRGL1
Alternative Gene Names
ALP, ALP1, FLJ22316
UniProt ID
Q7L266
Gene (Ensembl)
ENSG00000162174
Entrez Gene ID
Entrez gene: 80150
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000162174 on the Human Protein Atlas

Edqvist PH, Huvila J, Forsström B, Talve L, Carpén O, Salvesen HB, Krakstad C, Grénman S, Johannesson H, Ljungqvist O, Uhlén M, Pontén F, Auranen A

Loss of ASRGL1 expression is an independent biomarker for disease-specific survival in endometrioid endometrial carcinoma

Gynecol Oncol , April 07, 2015

PubMed ID: 25858696

Did we miss your publication?

Have you published using HPA029725? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ASRGL1 (AMAb90907)
Anti-ASRGL1 (HPA055572)
Corresponding Antigens
PrEST Antigen ASRGL1 (APrEST78796)

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