Enhanced Validation

Polyclonal Anti-ARHGAP45 Antibody

Rho GTPase activating protein 45
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human ARHGAP45
Alternative Gene Names
HA-1, HMHA1, KIAA0223
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
Rho GTPase activating protein 45
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000013220 (56%)

Mouse ENSMUSG00000035697 (54%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
Rho GTPase activating protein 45
Gene Name
Alternative Gene Names
HA-1, HMHA1, KIAA0223
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000180448 on the Human Protein Atlas

He L, Valignat M, Zhang L, Gelard L, Zhang F, Le Guen V, Audebert S, Camoin L, Fossum E, Bogen B, Wang H, Henri S, Roncagalli R, Theodoly O, Liang Y, Malissen M, Malissen B

ARHGAP45 controls naïve T‐ and B‐cell entry into lymph nodes and T‐cell progenitor thymus seeding

EMBO Rep , 2021 Mar 15; 22(4):e52196. Epub 2021 Mar 15

PubMed ID: 33719206 DOI: 10.15252/embr.202052196

de Kreuk BJ, Schaefer A, Anthony EC, Tol S, Fernandez-Borja M, Geerts D, Pool J, Hambach L, Goulmy E, Hordijk PL

The Human Minor Histocompatibility Antigen1 Is a RhoGAP

PLoS One , 2013; 8(9):e73962. Epub 2013 Sep 23

PubMed ID: 24086303 DOI: 10.1371/journal.pone.0073962

Did we miss your publication?

Have you published using HPA019816? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Corresponding Antigens


Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch