Polyclonal Anti-APTX Antibody
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000028411 (69%)
Rat ENSRNOG00000006582 (60%)
Recommended conditions:
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000137074 on the Human Protein Atlas
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Contribution from Diego Arango, Group of Molecular Oncology, Vall d'Hebron Hospital Research Institute — February 25, 2016
We have tested the Anti-APTX antibody (HPA064930) in formalin-fixed, paraffin embedded cells with forced overexpression of APTX. The results convincingly demonstrate the specificity of the HPA064930 antibody.
A. B.
The images show nuclear positivity in HCT116 colon cancer cells stably transfected with A) a constitutive APTX vector and B) a dox-dependent inducible APTX vector, compared to the control (HCT116 Parental and HCT116 -Dox).
Dilution 1:10 000 after citrate HIER.
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.
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