Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Contribution from Diego Arango, Group of Molecular Oncology, Vall d'Hebron Hospital Research Institute — February 25, 2016
We have tested the Anti-APTX antibody (HPA064930) in formalin-fixed, paraffin embedded cells with forced overexpression of APTX. The results convincingly demonstrate the specificity of the HPA064930 antibody.
The images show nuclear positivity in HCT116 colon cancer cells stably transfected with A) a constitutive APTX vector and B) a dox-dependent inducible APTX vector, compared to the control (HCT116 Parental and HCT116 -Dox).
Dilution 1:10 000 after citrate HIER.
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