Anti-ANXA1 Antibody
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000017469 (92%)
Mouse ENSMUSG00000024659 (89%)
Recommended conditions:
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-ANXA1 HPA011271.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000135046 on the Human Protein Atlas
Tumoral ANXA1 Is a Predictive Marker for Sunitinib Treatment of Renal Cancer Patients
J Cancer , 2017 Oct 23; 8(19):3975-3983. Epub 2017 Oct 23
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