Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000022244 (90%)
Rat ENSRNOG00000018662 (87%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-AMACR HPA019527.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer
Oncotarget , 2018 Nov 30; 9(94):36693-36704. Epub 2018 Nov 30
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