Anti-ALKBH5 Antibody

AlkB family member 5, RNA demethylase
Recommended Applications
Product Description
Polyclonal Antibody against Human ALKBH5
Alternative Gene Names
FLJ20308, OFOXD1
Price
$437.00
Product Number
HPA007196
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
AlkB family member 5, RNA demethylase
Target gene
ALKBH5
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
EKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVD
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000028341 (96%)

Mouse ENSMUSG00000042650 (95%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.4 µg/ml

Protocols
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
AlkB family member 5, RNA demethylase
Gene Name
ALKBH5
Alternative Gene Names
FLJ20308, OFOXD1
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000091542 on the Human Protein Atlas

Panneerdoss S, Eedunuri VK, Yadav P, Timilsina S, Rajamanickam S, Viswanadhapalli S, Abdelfattah N, Onyeagucha BC, Cui X, Lai Z, Mohammad TA, Gupta YK, Huang TH, Huang Y, Chen Y, Rao MK

Cross-talk among writers, readers, and erasers of m6A regulates cancer growth and progression

Sci Adv , 2018 Oct 3; 4(10):eaar8263. Epub 2018 Oct 3

PubMed ID: 30306128 DOI: 10.1126/sciadv.aar8263

Wang Y, Li Y, Yue M, Wang J, Kumar S, Wechsler-Reya RJ, Zhang Z, Ogawa Y, Kellis M, Duester G, Zhao JC

N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications

Nat Neurosci , 2018 Jan 15; 21(2):195-206. Epub 2018 Jan 15

PubMed ID: 29335608 DOI: 10.1038/s41593-017-0057-1

Yu J, Chen M, Huang H, Zhu J, Song H, Zhu J, Park J, Ji SJ

Dynamic m6A modification regulates local translation of mRNA in axons

Nucleic Acids Res , 2017 Nov 23; 46(3):1412-1423. Epub 2017 Nov 23

PubMed ID: 29186567 DOI: 10.1093/nar/gkx1182

Chang M, Lv H, Zhang W, Ma C, He X, Zhao S, Zhang ZW, Zeng YX, Song S, Niu Y, Tong WM

Region-specific RNA m6A methylation represents a new layer of control in the gene regulatory network in the mouse brain

Open Biol , 2017 Sep 20; 7(9):170166. Epub 2017 Sep 20

PubMed ID: 28931651 DOI: 10.1098/rsob.170166

Zhang S, Zhao BS, Zhou A, Lin K, Zheng S, Lu Z, Chen Y, Sulman EP, Xie K, Bögler O, Majumder S, He C, Huang S

The m6A Demethylase ALKBH5 Maintains Tumorigenicity of Glioblastoma Stem-Like Cells by Sustaining FOXM1 Expression and Cell Proliferation Program

Cancer Cell , 2017 Mar 23; 31(4):591-606.e6. Epub 2017 Mar 23

PubMed ID: 28344040 DOI: 10.1016/j.ccell.2017.02.013

Tirumuru N, Zhao BS, Lu W, Lu Z, He C, Wu L

N6-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression

eLife , 2016 Jul 2; 5:e15528. Epub 2016 Jul 2

PubMed ID: 27371828 DOI: 10.7554/eLife.15528

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Corresponding Antigens

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